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Transmembrane migration and inflammation tropism of hESCs loaded with NIR-NPs and Vis-NPs. A A scheme of the Boyden chamber transwell migration assay, where (I) stem cells are seeded into the upper chamber and (II) migrate toward the lower chamber containing LPS-treated microglia. B Representative fluorescence microscopy images of hESCs loaded with NIR/Vis-NPs (white/orange) that migrated to the lower chamber of the semipermeable membrane toward the LPS-treated microglia. Normal and <t>LPS-treated</t> <t>BV-2</t> were stained with DAPI (blue) and IBA-1 (green). C The bar chart of the relative number of migratory cells. Data are presented as M ± SD, with * p < 0.05 indicating statistically significant difference
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Transmembrane migration and inflammation tropism of hESCs loaded with NIR-NPs and Vis-NPs. A A scheme of the Boyden chamber transwell migration assay, where (I) stem cells are seeded into the upper chamber and (II) migrate toward the lower chamber containing LPS-treated microglia. B Representative fluorescence microscopy images of hESCs loaded with NIR/Vis-NPs (white/orange) that migrated to the lower chamber of the semipermeable membrane toward the LPS-treated microglia. Normal and LPS-treated BV-2 were stained with DAPI (blue) and IBA-1 (green). C The bar chart of the relative number of migratory cells. Data are presented as M ± SD, with * p < 0.05 indicating statistically significant difference

Journal: Stem Cell Research & Therapy

Article Title: Human stem cells loaded with visible and near-infrared fluorescent core–shell polymeric nanoparticles and revealing tropism toward inflammatory stimuli: in vitro evaluation

doi: 10.1186/s13287-025-04834-0

Figure Lengend Snippet: Transmembrane migration and inflammation tropism of hESCs loaded with NIR-NPs and Vis-NPs. A A scheme of the Boyden chamber transwell migration assay, where (I) stem cells are seeded into the upper chamber and (II) migrate toward the lower chamber containing LPS-treated microglia. B Representative fluorescence microscopy images of hESCs loaded with NIR/Vis-NPs (white/orange) that migrated to the lower chamber of the semipermeable membrane toward the LPS-treated microglia. Normal and LPS-treated BV-2 were stained with DAPI (blue) and IBA-1 (green). C The bar chart of the relative number of migratory cells. Data are presented as M ± SD, with * p < 0.05 indicating statistically significant difference

Article Snippet: The H1 hESC (ENCBS718AAA) and BV-2 cell lines were obtained from Ubigene Biosciences, Guangzhou Science City, China.

Techniques: Migration, Transwell Migration Assay, Fluorescence, Microscopy, Membrane, Staining